The process and practice of diagnosis: innovations in diagnostics for Lassa fever in Sierra Leone
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منابع مشابه
Early diagnosis of Lassa fever by reverse transcription-PCR.
We developed a method based on a coupled reverse transcription-PCR (RT-PCR) for the detection of Lassa virus using primers specific for regions of the S RNA segment which are well conserved between isolates from Sierra Leone, Liberia, and Nigeria. The specificity of the assay was confirmed by Southern blotting with a chemiluminescent probe. The assay was able to detect 1 to 10 copies of a plasm...
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